Analysis Note
Positive ControlMolt-4 cells
Application
Cell Inhibition Assays (1-5 µg/ml, see comments)r>Flow Cytometry (5 µg/ml)r>Frozen Sections (see comments)r>Immunoprecipitation (see comments)
General description
Protein A purified mouse monoclonal antibody. Recognizes the I domain of the ~180 kDa integrin α L subunit (CD11a).
Anti-CD11a, mouse monoclonal, clone 38, recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 cells. It is validated for FC, IP, IHC on frozen sections and for cell inhibition assays.
Recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 human tumor cells.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Also blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.
Holness, C.L., et al. 1995. J. Biol. Chem. 270, 877.Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.
Packaging
100 µg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
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